A Mechanism for Evolving Novel Plant Sesquiterpene Synthase Function

A Mechanism for Evolving Novel Plant Sesquiterpene Synthase Function.

Plant sesquiterpene synthases, a subset of the terpene synthase superfamily, are a mechanistically diverse family of enzymes capable of synthesizing hundreds of complex compounds with high regio- and stereospecificity and are of biological importance due to their role in plant defense mechanisms. In the current report we describe a large-scale, high-resolution phylogenetic analysis of ∼200 plan...

Sesquiterpene Synthase Gene and Protein

A Novel Objective Function for Directional Overcurrent Relays Coordination

Directional overcurrent relays (DOCRs) are widely used to protect power systems. For optimal coordination of DOCRs, several techniques have been proposed to solve this problem. A common way of optimal coordination of DOCRs is using evolutionary algorithms such as genetic algorithm (GA). In this paper, a novel strategy for DOCRs coordination is proposed. In the proposed strategy, a new objective...

A novel soil manganese mechanism drives plant

Loss of plant diversity with increased anthropogenic nitrogen (N) deposition in 40 grasslands has occurred globally. In most cases, competitive exclusion driven by preemption of 41 light or space is invoked as a key mechanism. Here, we provide evidence from a 9-year N 42 addition experiment for an alternative mechanism: differential sensitivity of forbs and grasses to 43 increased soil manganes...

Surrogate splicing for functional analysis of sesquiterpene synthase genes.

A method for the recovery of full-length cDNAs from predicted terpene synthase genes containing introns is described. The approach utilizes Agrobacterium-mediated transient expression coupled with a reverse transcription-polydeoxyribonucleotide chain reaction assay to facilitate expression cloning of processed transcripts. Subsequent expression of intronless cDNAs in a suitable prokaryotic host...